Journal: Arthritis Research & Therapy

Article Title: Best practices in DNA methylation: lessons from inflammatory bowel disease, psoriasis and ankylosing spondylitis

doi: 10.1186/s13075-019-1922-y

Figure Lengend Snippet: Summary table of methods for the detection of DNA methylation

Article Snippet: Somineni [ ] , CC , 164 paediatric CD , 74 paediatric HC , Peripheral blood , Illumina MethylationEPIC BeadChip , Previous findings were replicated including VMP1 , RPS6KA2 , ITGB2 , and TXK , alongside known therapeutic targets ( TNF , JAK3 , IL12B , IL23A , and IL1R1 ). 194 DMP had a strong evidence of genetic effect. The Crohn’s methylation signature was a powerful predictor of disease (AUC = 0.91), but had no prognostic utility..

Techniques: Methylation, Amplification, High Throughput Screening Assay, Sequencing, Methylation Sequencing, Selection, Genome Wide, Isolation, Immunoprecipitation, Methylated DNA Immunoprecipitation, Combined Bisulfite Restriction Analysis Assay, Ligation, Microarray, Mass Spectrometry, Fluorescence, SPR Assay

Journal: Arthritis Research & Therapy

Article Title: Best practices in DNA methylation: lessons from inflammatory bowel disease, psoriasis and ankylosing spondylitis

doi: 10.1186/s13075-019-1922-y

Figure Lengend Snippet: Seminal papers on DNA methylation in IBD, psoriasis and AS. Study design elements and key findings are outlined

Article Snippet: Somineni [ ] , CC , 164 paediatric CD , 74 paediatric HC , Peripheral blood , Illumina MethylationEPIC BeadChip , Previous findings were replicated including VMP1 , RPS6KA2 , ITGB2 , and TXK , alongside known therapeutic targets ( TNF , JAK3 , IL12B , IL23A , and IL1R1 ). 194 DMP had a strong evidence of genetic effect. The Crohn’s methylation signature was a powerful predictor of disease (AUC = 0.91), but had no prognostic utility..

Techniques: DNA Methylation Assay, Methylation, Methylated DNA Immunoprecipitation Sequencing, Expressing, Activity Assay